3B5P
Crystal structure of a cadd-like protein of unknown function (npun_f6505) from nostoc punctiforme pcc 73102 at 2.00 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000, 0.9797, 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.670, 78.510, 45.580 |
Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
Resolution | 28.736 - 2.000 |
R-factor | 0.169 |
Rwork | 0.167 |
R-free | 0.22500 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.434 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.736 | 28.736 | 2.060 |
High resolution limit [Å] | 2.000 | 4.270 | 2.000 |
Rmerge | 0.069 | 0.029 | 0.431 |
Number of reflections | 16846 | 3318 | 2237 |
<I/σ(I)> | 6.85 | 17.1 | 1.5 |
Completeness [%] | 91.9 | 94.9 | 66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | NANODROP, 0.2M Mg(OAc)2, 20.0% PEG 8000, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |