3B5K
Crystal structure of murine interleukin-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.193, 47.085, 55.053 |
| Unit cell angles | 90.00, 96.41, 90.00 |
Refinement procedure
| Resolution | 15.090 - 2.500 |
| R-factor | 0.21483 |
| Rwork | 0.213 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hul |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.915 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.700 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 6732 | |
| <I/σ(I)> | 13.7 | 3.9 |
| Completeness [%] | 96.8 | 94.6 |
| Redundancy | 6.35 | 6.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 26% PEG 4000, 0.1M Tris-HCl pH 8.5, 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
