3B5H
Crystal structure of the extracellular portion of HAb18G/CD147
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 126.481, 126.481, 169.926 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.600 - 2.800 |
R-factor | 0.245 |
Rwork | 0.243 |
R-free | 0.29400 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.460 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.106 | 0.586 |
Number of reflections | 34141 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 99.4 | 100 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.5M ammonium sulfate, 0.1M triSodium Citrate dihydrate pH 5.60, 1.0M Lithium Sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.5M ammonium sulfate, 0.1M triSodium Citrate dihydrate pH 5.60, 1.0M Lithium Sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.5M ammonium sulfate, 0.1M triSodium Citrate dihydrate pH 5.60, 1.0M Lithium Sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |