3B5A
Crystal Structure of a Minimally Hinged Hairpin Ribozyme Incorporating A38G mutation with a 2'OMe modification at the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.560, 93.560, 131.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.130 - 2.350 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.23800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2oue |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.080 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 14645 | |
| <I/σ(I)> | 25.6 | 3.9 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 11.7 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | PEG 2K MME, lithium sulfate, Tris, spermidine, cobalt hexaammine, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2K MME | ||
| 2 | 1 | 1 | Li2SO4 | ||
| 3 | 1 | 1 | Tris | ||
| 4 | 1 | 1 | spermidine | ||
| 5 | 1 | 1 | cobalt hexaammine | ||
| 6 | 1 | 2 | PEG 2K MME | ||
| 7 | 1 | 2 | Li2SO4 | ||
| 8 | 1 | 2 | Tris | ||
| 9 | 1 | 2 | spermidine |
