3B58
Minimally Junctioned Hairpin Ribozyme Incorporates A38G Mutation and a 2',5'-Phosphodiester Linkage at the Active Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.370, 93.370, 132.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.800 - 2.650 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.26200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2p7f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 10328 | |
| <I/σ(I)> | 20.7 | 3.8 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 10.2 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 2K MME, lithium sulfate, HEPES, spermidine, cobalt hexaammine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2K MME | ||
| 2 | 1 | 1 | Li2SO4 | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | spermidine | ||
| 5 | 1 | 1 | cobalt hexaammine | ||
| 6 | 1 | 2 | PEG 2K MME | ||
| 7 | 1 | 2 | Li2SO4 | ||
| 8 | 1 | 2 | HEPES | ||
| 9 | 1 | 2 | spermidine |
