3B4U
Crystal structure of dihydrodipicolinate synthase from Agrobacterium tumefaciens str. C58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.116, 49.442, 69.084 |
| Unit cell angles | 79.35, 89.06, 83.94 |
Refinement procedure
| Resolution | 67.880 - 1.200 |
| R-factor | 0.12846 |
| Rwork | 0.127 |
| R-free | 0.15559 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.880 | 1.231 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.077 | 0.274 |
| Number of reflections | 157988 | |
| <I/σ(I)> | 17.89 | 3 |
| Completeness [%] | 93.2 | 72.49 |
| Redundancy | 4.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Mg formate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
