3B4Q
Crystal structure of a conserved protein domain (unknown function) from Corynebacterium diphtheriae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.658, 103.857, 26.329 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.520 - 1.550 |
R-factor | 0.18621 |
Rwork | 0.185 |
R-free | 0.21580 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.581 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.520 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.069 | 0.446 |
Number of reflections | 25547 | |
<I/σ(I)> | 38 | 2.4 |
Completeness [%] | 94.6 | 65.8 |
Redundancy | 6.4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Li2SO4, 0.1M Tris-HCl, 30% PEG 3000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |