3B4M
Crystal Structure of Human PABPN1 RRM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-28 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 31 |
| Unit cell lengths | 59.338, 59.338, 80.599 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.580 - 2.820 |
| R-factor | 0.22932 |
| Rwork | 0.226 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b4d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.252 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.582 | 2.920 |
| High resolution limit [Å] | 2.816 | 2.816 |
| Rmerge | 0.063 | |
| Number of reflections | 7694 | |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | 0.1M HEPES, 4.2M sodium chloride, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
