3B4A
T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 177.154, 41.692, 77.699 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| Rwork | 0.246 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.182 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.083 | 0.933 |
| Number of reflections | 16141 | |
| <I/σ(I)> | 25.4 | 2.7 |
| Completeness [%] | 97.2 | 93.6 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | GROWN FROM: 12% PEG 4000, 1M LICL, 100MM MES-NAOH PH 5.4, 5MM MGCL2, STABILIZED IN: 24% PEG 4000, 1.5M LICL, 30MM MGCL2, 100MM MES-NAOH PH 5.5, 20MM GLUCOSAMINE-6-PHOSPHATE, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 2 | PEG 4000 | ||
| 10 | 1 | 1 | PEG 4000 | ||
| 11 | 1 | 1 | LICL | ||
| 12 | 1 | 1 | MES-NAOH PH 5.4 | ||
| 13 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 2 | LICL | ||
| 3 | 1 | 2 | MES-NAOH PH 5.4 | ||
| 4 | 1 | 2 | MGCL2 | ||
| 5 | 1 | 3 | PEG 4000 | ||
| 6 | 1 | 3 | LICL | ||
| 7 | 1 | 3 | MGCL2 | ||
| 8 | 1 | 3 | MES-NAOH PH 5.5 | ||
| 9 | 1 | 3 | GLUCOSAMINE-6-PHOSPHATE |
