3B49
Crystal structure of an uncharacterized conserved protein from Listeria innocua
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.506, 68.108, 69.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.19800 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.077 | 0.369 |
| Number of reflections | 31607 | |
| <I/σ(I)> | 26.6 | 2.1 |
| Completeness [%] | 97.2 | 82.2 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1M HEPES pH 7.5, 1.4M tri-Sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
