3B48
Crystal structure of unknown function protein EF1359
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.080, 102.112, 60.454 |
Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
Resolution | 44.990 - 2.210 |
R-factor | 0.20304 |
Rwork | 0.201 |
R-free | 0.24630 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.457 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.103 | 0.422 |
Number of reflections | 34382 | |
<I/σ(I)> | 24.72 | 2.73 |
Completeness [%] | 95.3 | 83.4 |
Redundancy | 6.4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.05M MgCl2, 0.1M HEPES, 30% PEG MME550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |