3B46
Crystal Structure of Bna3p, a Putative Kynurenine Aminotransferase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.780, 66.520, 115.200 |
| Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
| Resolution | 28.800 - 2.000 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w7l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.065 | 0.255 |
| Number of reflections | 60609 | |
| <I/σ(I)> | 36.9 | 4.7 |
| Completeness [%] | 98.6 | 93.6 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 10 mM Tris, 50 mM NaCl, 14 mM mercaptoethanol, 0.025 mM PLP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
