3B45
Crystal structure of GlpG at 1.9A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 1.0809 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 111.000, 111.000, 129.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| Rwork | 0.215 |
| R-free | 0.22800 |
| Starting model (for MR) | 2ic8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.657 |
| Number of reflections | 23768 | |
| <I/σ(I)> | 18.6 | 1.2 |
| Completeness [%] | 97.9 | 89.8 |
| Redundancy | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 1.5M NH4Cl, 100mM Bis-Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
