3B39
Structure of the DnaG primase catalytic domain bound to ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-01-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.111 |
Spacegroup name | P 62 |
Unit cell lengths | 136.829, 136.829, 71.071 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.350 |
R-factor | 0.21398 |
Rwork | 0.212 |
R-free | 0.25443 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dde |
RMSD bond length | 0.011 |
RMSD bond angle | 1.185 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.065 | 0.458 |
Number of reflections | 30546 | |
<I/σ(I)> | 14.8 | 2.2 |
Completeness [%] | 95.7 | 97.3 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 21% PEG 4000, 100 mM ammonium acetate, 50 mM sodium acetate (pH 5.0), VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | ammonium acetate | ||
3 | 1 | 1 | sodium acetate | ||
4 | 1 | 2 | PEG 4000 | ||
5 | 1 | 2 | ammonium acetate | ||
6 | 1 | 2 | sodium acetate |