3B36
Structure of M26L DJ-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2007-09-10 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.818, 74.818, 75.264 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.820 - 1.500 |
R-factor | 0.16633 |
Rwork | 0.166 |
R-free | 0.17486 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p5f |
RMSD bond length | 0.007 |
RMSD bond angle | 1.165 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.820 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.069 | 0.160 |
Number of reflections | 39440 | |
<I/σ(I)> | 36.8 | 13 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 9.8 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 30% PEG 3000, 100 mM HEPES, 200 mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |