3B32
Crystal Structure of Calcium-Saturated Calmodulin N-Terminal Domain Fragment, Residues 1-75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.352, 66.096, 57.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.240 - 1.600 |
| R-factor | 0.20069 |
| Rwork | 0.197 |
| R-free | 0.26916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cln |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.226 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.240 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.049 | 0.248 |
| Number of reflections | 11740 | |
| <I/σ(I)> | 19.4 | 3.3 |
| Completeness [%] | 97.8 | 94.2 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 100 mM Na acetate, 0.01% Na azide, 21.25% PEG 8000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
