3B31
Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.724, 58.724, 98.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.200 - 2.400 |
Rwork | 0.239 |
R-free | 0.26860 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.350 |
Data reduction software | ELVES |
Data scaling software | CCP4 |
Phasing software | PHENIX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.200 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | |
Number of reflections | 7225 | |
<I/σ(I)> | 27.2 | 2 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 31.9 | 18.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 1.4 M lithium sulfate, 40 mM magnesium acetate, 50 mM HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 1.4 M lithium sulfate, 40 mM magnesium acetate, 50 mM HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | lithium sulfate | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | HEPES-NaOH | ||
4 | 1 | 2 | lithium sulfate | ||
5 | 1 | 2 | magnesium acetate | ||
6 | 1 | 2 | HEPES-NaOH |