3B31
Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.724, 58.724, 98.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.200 - 2.400 |
| Rwork | 0.239 |
| R-free | 0.26860 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.350 |
| Data reduction software | ELVES |
| Data scaling software | CCP4 |
| Phasing software | PHENIX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.200 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | |
| Number of reflections | 7225 | |
| <I/σ(I)> | 27.2 | 2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 31.9 | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 1.4 M lithium sulfate, 40 mM magnesium acetate, 50 mM HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | 1.4 M lithium sulfate, 40 mM magnesium acetate, 50 mM HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | lithium sulfate | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | HEPES-NaOH | ||
| 4 | 1 | 2 | lithium sulfate | ||
| 5 | 1 | 2 | magnesium acetate | ||
| 6 | 1 | 2 | HEPES-NaOH |
