3B2C
Crystal structure of the collagen triple helix model [{PRO-HYP(R)-GLY}4-{HYP(S)-Pro-GLY}2-{PRO-HYP(R)-GLY}4]3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-14 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.80 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.797, 26.152, 84.743 |
| Unit cell angles | 90.00, 92.48, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.360 |
| R-factor | 0.20507 |
| Rwork | 0.203 |
| R-free | 0.24792 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.756 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Number of reflections | 42284 | |
| Completeness [%] | 98.9 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 100mM Bicine buffer, 20% (w/v) Polyethylene glycol monomethyl ether 550, 100mM sodium chloride, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
