3B1S
Crystal structure of the cytoplasmic domain of FlhB from Aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.600, 33.750, 122.370 |
Unit cell angles | 90.00, 107.78, 90.00 |
Refinement procedure
Resolution | 29.750 - 2.550 |
R-factor | 0.24228 |
Rwork | 0.241 |
R-free | 0.26195 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.844 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.760 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 14910 | |
Completeness [%] | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.2M sodium thiocyanate, 20% PEG 3350, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
2 |