3B1B
The unique structure of wild type carbonic anhydrase alpha-CA1 from Chlamydomonas reinhardtii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00, 1.28254, 1.28297 |
Spacegroup name | P 65 |
Unit cell lengths | 134.300, 134.300, 120.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.300 - 1.880 |
R-factor | 0.18886 |
Rwork | 0.187 |
R-free | 0.21757 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.546 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.082 | 0.314 |
Number of reflections | 99548 | |
<I/σ(I)> | 60 | 8.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.6 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.0-2.0M Ammonium sulfate, 100mM Tris-HCl (pH8.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |