3B0S
Crystal Structure of (Gly-Pro-Hyp)9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.310, 26.161, 74.507 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.450 |
| Rwork | 0.170 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2drt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.018 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.073 | 0.191 |
| Number of reflections | 20281 | |
| <I/σ(I)> | 4.9 | 3.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 22% PEG 200, 0.1M HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
