3B0K
Crystal structure of alpha-lactalbumin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.631, 88.210, 43.921 |
Unit cell angles | 90.00, 96.94, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.18323 |
Rwork | 0.180 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hfy |
RMSD bond length | 0.022 |
RMSD bond angle | 2.113 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 30569 | |
Completeness [%] | 97.2 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 26% PEG 4000, 0.2M ammonium sulphate, 0.1M Na-acetate, 24 mg/ml protein, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |