3B0I
Crystal structure of recombinant human alpha lactalbumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.702, 33.156, 46.148 |
| Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.2118 |
| Rwork | 0.209 |
| R-free | 0.25602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b9o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.415 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 8750 | |
| Completeness [%] | 99.1 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 2.0M ammonium sulfate, 0.01M CaCl2, 0.1M MES, 28mg/ml protein, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
