3B0F

Crystal structure of the UBA domain of p62 and its interaction with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	72.967, 72.967, 32.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.400
R-factor	0.17167
Rwork	0.170
R-free	0.20054
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.697
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.570	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.048	0.356
Number of reflections	17371
<I/σ(I)>	13.1
Completeness [%]	97.0	82.5
Redundancy	13.1	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100mM Citrate buffer pH5.0, 1.8M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	100mM Citrate buffer pH5.0, 1.8M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K