3B0C

Crystal structure of the chicken CENP-T histone fold/CENP-W complex, crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	54.933, 54.933, 122.463
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.980 - 2.201
R-factor	0.229
Rwork	0.220
R-free	0.26870
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.358
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.980	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	10722
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100mM Citrate-NaOH pH 5.0, 30% PEG 600, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	100mM Citrate-NaOH pH 5.0, 30% PEG 600, VAPOR DIFFUSION, HANGING DROP, temperature 293K