3B0C
Crystal structure of the chicken CENP-T histone fold/CENP-W complex, crystal form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 54.933, 54.933, 122.463 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.980 - 2.201 |
R-factor | 0.229 |
Rwork | 0.220 |
R-free | 0.26870 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.358 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.980 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 10722 | |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100mM Citrate-NaOH pH 5.0, 30% PEG 600, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100mM Citrate-NaOH pH 5.0, 30% PEG 600, VAPOR DIFFUSION, HANGING DROP, temperature 293K |