3B09
Crystal structure of the N-domain of FKBP22 from Shewanella sp. SIB1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97888, 0.97919, 0.96500 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 39.703, 39.703, 101.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.380 - 1.900 |
| R-factor | 0.23386 |
| Rwork | 0.232 |
| R-free | 0.27597 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.114 | 0.423 |
| Number of reflections | 7712 | |
| <I/σ(I)> | 18.2 | 2.6 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277 | 0.1M Na/K Phosphate, 20% PEG 1000, 0.2 M NaCl, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
