3AZY
Crystal structure of the laminarinase catalytic domain from Thermotoga maritima MSB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 |
Unit cell lengths | 120.420, 120.420, 107.195 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.600 - 1.650 |
R-factor | 0.16436 |
Rwork | 0.163 |
R-free | 0.19226 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3azx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.422 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.057 | 0.441 |
Number of reflections | 183216 | |
<I/σ(I)> | 26.8 | 3.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 41-46% (w/v) polypropylenglycol P400, 0.05-0.2M (NH4)2SO4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |