3AZX
Crystal structure of the laminarinase catalytic domain from Thermotoga maritima MSB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.567, 56.152, 126.431 |
| Unit cell angles | 90.00, 105.26, 90.00 |
Refinement procedure
| Resolution | 25.600 - 1.800 |
| R-factor | 0.20052 |
| Rwork | 0.198 |
| R-free | 0.24788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.086 | 0.496 |
| Number of reflections | 62313 | |
| <I/σ(I)> | 16.4 | 2.4 |
| Completeness [%] | 98.8 | 95.8 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 10-15% (w/v) PEG 8000, 0.25-0.45 M KH2PO4, 20% glycerol, 10mM CaCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
