3AZW
Crystal structure of the receptor binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-28 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.958, 141.550, 162.039 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.220 - 2.990 |
R-factor | 0.25691 |
Rwork | 0.255 |
R-free | 0.29096 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.026 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.6.0091) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.150 |
High resolution limit [Å] | 2.990 | 2.990 |
Number of reflections | 27614 | |
<I/σ(I)> | 2.69 | |
Completeness [%] | 99.3 | 95.6 |
Redundancy | 8.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1M MES, 1.6M magnesium sulfate, 1M sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 293K |