3AZU
X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.740, 99.150, 47.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.100 |
R-factor | 0.163 * |
Rwork | 0.163 |
RMSD bond length | 0.012 |
RMSD bond angle | 2.870 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | |
High resolution limit [Å] | 2.090 * | 2.090 * |
Rmerge | 0.097 * | |
Total number of observations | 99146 * | |
Number of reflections | 22537 * | |
Completeness [%] | 66.6 * | 21.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 3.5-3.8 (M) | |
2 | 1 | reservoir | lithium nitrate | 0.5 (M) | |
3 | 1 | reservoir | acetate | 0.1 (M) | can be replaced with MES |
4 | 1 | reservoir | protein | 12-17 (mg/ml) |