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3AZG

Crystal Structure of Human Nucleosome Core Particle Containing H3K115Q mutation

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL41XU
Synchrotron siteSPring-8
BeamlineBL41XU
Temperature [K]100
Detector technologyCCD
Collection date2009-11-23
DetectorADSC QUANTUM 315
Wavelength(s)1.0000
Spacegroup nameP 21 21 21
Unit cell lengths106.527, 109.772, 182.365
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution39.160 - 2.400
Rwork0.226
R-free0.26900
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2cv5
RMSD bond length0.008
RMSD bond angle1.200
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareCNS (1.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.490
High resolution limit [Å]2.4002.400
Number of reflections84116
<I/σ(I)>2.9
Completeness [%]99.799.5
Redundancy7.26.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6293POTASSIUM CACODYLATE, POTASSIUM CHLORIDE, MANGANESE CHLORIDE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

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