3AZC
Crystal structure of the soluble part of cytochrome b6f complex iron-sulfur subunit from Thermosynechococcus elongatus BP-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 40 |
Detector technology | CCD |
Collection date | 2011-02-02 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.65 |
Spacegroup name | P 65 |
Unit cell lengths | 91.324, 91.324, 43.342 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.610 - 2.000 |
Rwork | 0.197 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e74 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 24682 | |
<I/σ(I)> | 32.2 | 2.1 |
Completeness [%] | 87.4 | 57.1 |
Redundancy | 7.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Na-HEPES, 10% 2-propanol, 20% PEG 3000, 15% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |