3AYR
GH5 endoglucanase EglA from a ruminal fungus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 83.396, 83.396, 225.085 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17269 |
| Rwork | 0.170 |
| R-free | 0.21575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.437 |
| Number of reflections | 31439 | |
| <I/σ(I)> | 54.6 | 8.8 |
| Completeness [%] | 97.5 | 97.1 |
| Redundancy | 39.5 | 42.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1.5M sodium citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
