3AYQ

Crystal structure of inhibitor bound lysozyme from Meretrix lusoria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	42.123, 42.123, 122.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.090 - 1.770
Rwork	0.228
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ab6
RMSD bond length	0.004
RMSD bond angle	1.299
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.770
Number of reflections	11424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	35% 2-propanol, 0.1M Citrate buffer (pH5.5), 5% PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 293K