3AYN
Crystal structure of squid isorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 62 |
| Unit cell lengths | 122.540, 122.540, 158.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| Rwork | 0.180 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS (1.2) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.260 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.086 | 0.527 |
| Number of reflections | 36893 | |
| <I/σ(I)> | 10.9 | 2.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 3.2M ammonium sulfate, 20mM MES, 50mM EDTA, 10mM beta-mercaptoethanol , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
