3AY5
Crystal structure of HHM (human homologue of murine maternal Id-like molecule)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.031, 105.031, 124.266 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.624 - 2.500 |
R-factor | 0.2239 |
Rwork | 0.222 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.029 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.046 | 0.023 | 0.371 |
Number of reflections | 25654 | ||
<I/σ(I)> | 26 | ||
Completeness [%] | 91.8 | 74.9 | 91.6 |
Redundancy | 6.8 | 8.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100mM imidazole, 500mM (NH4)2HPO4, 100mM NaCl, pH 8.0, vapor diffusion, sitting drop, temperature 293K |