3AXY
Structure of Florigen Activation Complex Consisting of Rice Florigen Hd3a, 14-3-3 Protein GF14 and Rice FD Homolog OsFD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 76.735, 96.647, 99.510 |
Unit cell angles | 68.23, 87.90, 77.94 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.23079 |
Rwork | 0.227 |
R-free | 0.27626 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB Code 1WKP for Hd3a 2O98 for GF14c |
RMSD bond length | 0.006 |
RMSD bond angle | 0.871 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 101603 | |
<I/σ(I)> | 12.4 | 3.3 |
Completeness [%] | 98.1 | 97.1 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES (pH 7.5), 0.2M ammonium sulfate, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |