3AXT

Complex structure of tRNA methyltransferase Trm1 from Aquifex aeolicus with S-adenosyl-L-Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	54.438, 140.928, 119.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.415 - 2.491
R-factor	0.1871
Rwork	0.185
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ejt
RMSD bond length	0.002
RMSD bond angle	0.560
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.491	2.500
Number of reflections	16410
Completeness [%]	99.8	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.1M Na-Citrate, 2M (NH4)2SO4, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K