3AXM
Structure of rice Rubisco in complex with 6PG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-11-23 |
Detector | OTHERS |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.408, 199.624, 111.169 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 74.260 - 1.650 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.260 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.056 | 0.311 |
Number of reflections | 511171 | |
Completeness [%] | 89.3 | 91.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 75mM HEPES-KOH(pH 7.75), 9% PEG4000, 25% glycerol, VAPOR DIFFUSION, temperature 293K |