3AXF
Perrhenate binding to A11C/R153C ModA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 77.2 |
Detector technology | IMAGE PLATE |
Collection date | 2010-04-27 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.751, 77.458, 163.033 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.2066 |
Rwork | 0.204 |
R-free | 0.25788 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.916 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 43585 | |
<I/σ(I)> | 39.6 | 8.9 |
Completeness [%] | 56.4 | 56.4 |
Redundancy | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 26% PEG8000, 0.1 M sodium acetate, 2.5 mM sodium perrhenate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |