3AXF
Perrhenate binding to A11C/R153C ModA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 77.2 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-27 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.751, 77.458, 163.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.2066 |
| Rwork | 0.204 |
| R-free | 0.25788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 43585 | |
| <I/σ(I)> | 39.6 | 8.9 |
| Completeness [%] | 56.4 | 56.4 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 26% PEG8000, 0.1 M sodium acetate, 2.5 mM sodium perrhenate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
