3AXD
The truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase V18Y/W203Y in apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2010-10-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.391, 69.963, 81.242 |
| Unit cell angles | 90.00, 100.98, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.530 |
| Rwork | 0.173 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zm1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.041 | 0.190 |
| Number of reflections | 66704 | |
| <I/σ(I)> | 33.5 | 7.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Tris-HCl, pH 7.5, 0.3M Calcium actetate, 29% PEG5KMME, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
