3AXC
Crystal structure of linear diubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-06-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 33.261, 35.314, 35.841 |
Unit cell angles | 91.79, 112.85, 112.88 |
Refinement procedure
Resolution | 32.260 - 2.190 |
R-factor | 0.20933 |
Rwork | 0.206 |
R-free | 0.27798 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.013 |
RMSD bond angle | 1.509 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.260 | 2.244 |
High resolution limit [Å] | 2.190 | 2.190 |
Number of reflections | 6959 | |
Completeness [%] | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | 30% PEG 4000, 0.1M Tris-HCl, 0.1M MgCl2, 30% Dioxane, 30% Isopropanol , pH 7.5, VAPOR DIFFUSION, temperature 289K |