3AXB
Structure of a dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97884, 0.97910, 0.96388 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 60.182, 60.182, 274.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.150 - 1.920 |
R-factor | 0.1886 |
Rwork | 0.188 |
R-free | 0.20470 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.486 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.920 |
Number of reflections | 73214 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop | 5.6 | 293 | PEG4000, ammonium acetate, tri-sodium citrate dihydrate, pH 5.6, sitting drop, temperature 293K |