3AX7
Bovine Xanthine Oxidase, protease cleaved form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.477, 73.151, 146.267 |
| Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
| Resolution | 36.370 - 2.340 |
| R-factor | 0.19658 |
| Rwork | 0.194 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.605 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.370 |
| High resolution limit [Å] | 2.340 |
| Number of reflections | 104898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 293 | 6-9% PEG 4000, 50mM potassium phosphate, 1mM salicylate, 0.2mM EDTA, 5mM DTT, 30% glycerol, pH 7.4, VAPOR DIFFUSION, temperature 293K |
