3AVU

Structure of viral RNA polymerase complex 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Collection date	2010-06-29
Spacegroup name	C 2 2 21
Unit cell lengths	139.000, 255.470, 101.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.973 - 2.907
R-factor	0.222
Rwork	0.219
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3agp
RMSD bond length	0.010
RMSD bond angle	1.342
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Number of reflections	39345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			VAPOR DIFFUSION, SITTING DROP