Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.419, 78.471, 143.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.723 - 2.000 |
| R-factor | 0.18757 |
| Rwork | 0.185 |
| R-free | 0.23904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DTQ |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.672 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.677 |
| Number of reflections | 38712 | |
| <I/σ(I)> | 12.5 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7 | 277 | deionized water, 20% 2,3-butanediol, pH 7.0, MICRODIALYSIS, temperature 277K |
