3AV6
Crystal structure of mouse DNA methyltransferase 1 with AdoMet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 134.995, 97.139, 130.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.140 - 3.090 |
| R-factor | 0.1945 |
| Rwork | 0.191 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3av4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.090 | 8.400 | 3.100 |
| Rmerge | 0.086 | 0.034 | 0.331 |
| Number of reflections | 32007 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.8 | 97.3 | 100 |
| Redundancy | 4.8 | 4.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 297 | PEG 3350, NaCl, TCEP, pH 9.0, vapor diffusion, temperature 297K |
