3AV3
Crystal structure of glycinamide ribonucleotide transformylase 1 from Geobacillus kaustophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-15 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97944, 0.90000, 0.97984 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.861, 117.408, 67.251 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.770 - 1.700 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.21400 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.045 | 0.115 |
Number of reflections | 43718 | |
<I/σ(I)> | 14.7 | |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | Bis-Tris 0.1M pH 6.4, 0.5M Sodium Fluoride, PEG 3350 25%, Mg Chloride 0.1M, VAPOR DIFFUSION, SITTING DROP |