3AUK
Crystal structure of a lipase from Geobacillus sp. SBS-4S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.131, 71.745, 126.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.150 - 1.660 |
| R-factor | 0.16371 |
| Rwork | 0.162 |
| R-free | 0.19031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1KUO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.981 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.063 | 0.472 |
| Number of reflections | 59613 | |
| <I/σ(I)> | 23.2 | 2.7 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M imidazole pH 8.0, 10% PEG 8000, 0.2M Ca-acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
