3AU3
Crystal structure of armadillo repeat domain of APC
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97897, 0.97934, 0.964 |
| Spacegroup name | P 61 |
| Unit cell lengths | 114.327, 114.327, 61.821 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.510 - 2.100 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 27064 | |
| <I/σ(I)> | 29.5 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.2 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100mM sodium acetate, 1.25M lithium citrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
